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The International Journal of Oral & Maxillofacial Implants
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Int J Oral Maxillofac Implants 30 (2015), No. 3     19. May 2015
Int J Oral Maxillofac Implants 30 (2015), No. 3  (19.05.2015)

Page 512-518, doi:10.11607/jomi.jomi.3701a, PubMed:26009901


Biochemical Responses of Anodized Titanium Implants with a Poly(lactide-co-glycolide)/Bone Morphogenic Protein-2 Submicron Particle Coating. Part 1: An In Vitro Study
Yoo, Soo-Yeon / Kim, Seong-Kyun / Heo, Seong-Joo / Koak, Jai-Young / Lee, Joo-Hee / Park, Ji-Man
Purpose: This study was conducted to examine the effects of coating poly(D,L-lactide-co-glycolide) (PLGA)/ recombinant human bone morphogenetic protein-2 (rhBMP-2) submicron particles by electrospray onto titanium (Ti) implants on the proliferation and differentiation capacity of mesenchymal stem cells. In addition, the duration of rhBMP-2 release was investigated.
Materials and Methods: Ti disks were fabricated and divided into four groups as follows. Group C (control) was anodized at 300 V, group P was anodized and then coated with 0.2% PLGA, group B1 was anodized and then coated with PLGA/rhBMP-2 submicron particles at 200 ng per disk, and group B2 was anodized and then coated with PLGA/rhBMP-2 submicron particles at 600 ng per disk. The topography of the PLGA/rhBMP-2-coated Ti surfaces was examined, and proliferation assays, alkaline phosphatase activity tests, and rhBMP-2 releasing tests were conducted in vitro.
Results: There was no difference in the roughness of control Ti disks and Ti disks coated with PLGA/rhBMP-2 submicron particles by electrospray. The proliferation of mesenchymal stem cells increased over time; at 7 days, cell proliferation on the Ti disks coated with rhBMP-2 was significantly higher than the other groups. Anodized Ti disks coated with PLGA/rhBMP-2 (groups B1 and B2) released rhBMP-2 for approximately 21 days. In accordance with the amount of rhBMP-2 released from the PLGA/rhBMP-2 construct, the differentiation capacity of mesenchymal stem cells on the PLGA/rhBMP-2-coated disks was increased significantly.
Conclusion: Submicron PLGA/rhBMP-2 coating on Ti implants by electrospray facilitated cell proliferation and differentiation, which is important for early healing and integration of implants.

Keywords: anodized implant, coating, differentiation, electrospray, mesenchymal stem cells, poly(D,L-lactideco- glycolide), proliferation, recombinant human bone morphogenetic proteins