We are using cookies to implement functions like login, shopping cart or language selection for this website. Furthermore we use Google Analytics to create anonymized statistical reports of the usage which creates Cookies too. You will find more information in our privacy policy.
OK, I agree I do not want Google Analytics-Cookies
The International Journal of Oral & Maxillofacial Implants



Forgotten password?


Int J Oral Maxillofac Implants 23 (2008), No. 6     15. Nov. 2008
Int J Oral Maxillofac Implants 23 (2008), No. 6  (15.11.2008)

Page 1013-1019, PubMed:19216269

Bone Formation on Apatite-Coated Titanium Incorporated with Bone Morphogenetic Protein and Heparin
Kodama, Takashi / Goto, Tetsuya / Miyazaki, Toshiki / Takahashi, Tetsu
Purpose: Bone morphogenetic proteins (BMPs) strongly induce osteogenesis and are enhanced by heparin. In this study, a potent osteoinductive material was developed by coating the surface of titanium with apatite incorporated with BMP and heparin.
Materials and Methods: Titanium samples were treated with a 5 N NaOH solution and heated at 600°C for 24 hours. The treated titanium was soaked in simulated body fluid (SBF) for 4 days and additionally soaked in SBF containing recombinant human BMP-2 (rhBMP-2;1,000 ng/mL) with or without heparin (30 µg/mL) for 3 days at 37°C. The surfaces of each sample were examined with scanning electron microscopy. The presence of rhBMP-2 on the surface of the apatite-coated titanium was examined using the immunogold method. MC3T3-E1 osteoblast-like cells were cultured on the surface of each sample. The number and morphology of the adherent cells, alkaline phosphatase activity, and osteocalcin mRNA expression were examined.
Results: Apatite was formed on the surface of alkaline heat-treated titanium after soaking in SBF for 7 days. The presence of rhBMP-2 was confirmed by the distribution of BMP-positive immunogold particles. The incorporation of (>= 3 µg/mL) heparin significantly increased alkaline phosphatase activity and osteocalcin mRNA expression in the cells on apatite-coated titanium containing rhBMP-2.
Conclusion: These findings demonstrate that heparin enhanced BMP-2-induced osteogenesis on apatite-coated titanium without the loss of BMP-2 activity. The combination of BMP-2 and heparin was effective even in the thin apatite layer formed on titanium using the alkaline heat treatment method.

Keywords: BMP-2, heparin, osteogenesis, simulated body fluid, titanium