We are using cookies to implement functions like login, shopping cart or language selection for this website. Furthermore we use Google Analytics to create anonymized statistical reports of the usage which creates Cookies too. You will find more information in our privacy policy.
OK, I agree I do not want Google Analytics-Cookies
The International Journal of Oral & Maxillofacial Implants



Forgotten password?


Int J Oral Maxillofac Implants 21 (2006), No. 4     15. July 2006
Int J Oral Maxillofac Implants 21 (2006), No. 4  (15.07.2006)

Page 526-534, PubMed:16955602

Tissue Engineering of a Periodontal Ligament-Alveolar Bone Graft Construct
Chou, Ai Mei / Sae-Lim, Varawan / Hutmacher, Dietmar W. / Lim, Tit Meng
Purpose: This paper reports on a 2-phase study of a novel membrane-scaffold graft construct, its ability to support periodontal ligament fibroblast (PDLF) and alveolar osteoblast (AO) growth in vitro, and its use for tissue engineering a PDL-AO interface in vivo.
Materials and Methods: Human PDLFs were seeded onto perforated poly(e-caprolactone) membranes (n = 30) at 78,000 cells/cm2; human AOs were seeded on poly(e-caprolactone) scaffolds (n = 30) with fibrin glue at 625,000 cells/cm3. Cell attachment, morphology, viability, and metabolic activity were monitored for 3 weeks in vitro. Subsequently, cell-seeded membrane-scaffold constructs (experimental group, n = 9) and nonseeded constructs (control group, n = 4) assembled with fibrin glue were implanted subcutaneously into 7 athymic mice for 4 weeks.
Results: PDLFs formed confluent layers on membranes, whereas AOs produced mineralized matrices within scaffolds upon osteoinduction in vitro. Well-vascularized tissue formation was observed after implantation. Integration at the membrane-scaffold interface was enhanced in the experimental group. Type I collagen, type III collagen, fibronectin, and vitronectin were found adjacent to membranes and within constructs. Bone sialoprotein expression and bone formation were undetectable.
Discussion: Membrane perforation and scaffold porosity facilitated tissue integration and vascularization at the construct-recipient site. However, the interaction between PDLF and AO could have interfered with osteogenesis at the interface of soft and mineralizing tissues.
Conclusions: Both matrices supported PDLF and AO attachment and proliferation in vitro. The membrane-scaffold construct facilitated tissue growth and vascularization while providing strength and form in vivo.

Keywords: alveolar osteoblasts, graft construct, human periodontal ligament fibroblasts, poly (e-caprolactone)