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The International Journal of Oral & Maxillofacial Implants



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Int J Oral Maxillofac Implants 20 (2005), No. 6     15. Nov. 2005
Int J Oral Maxillofac Implants 20 (2005), No. 6  (15.11.2005)

Page 882-890

Image-Based Extracorporeal Tissue Engineering of Individualized Bone Constructs
Meyer, Ulrich / Büchter, Andre / Hohoff, Ariane / Stoffels, Elke / Szuwart, Thomas / Runte, Christoph / Dirksen, Dieter / Wiesmann, Hans-Peter
Purpose: Computer-aided technologies have been recently employed for use in extracorporeal bone tissue engineering strategies. In this pilot animal experimental study, the intention was to test whether autologous osteoblast-like cells cultured in vitro on individualized scaffolds can be used to support bone regeneration in a clinical environment.
Materials and Methods: For this purpose, mandibular bone defects were surgically introduced into the mandibles of minipigs and the scaffold of the defect site was modeled by computer-aided design/computer-aided manufacturing technique. Autologous bone cells from porcine calvaria were harvested from minipigs and grown in culture. Cells were seeded on scaffolds generated by rapid prototyping of polylactic acid/polyglycolic acid copolymers. The defects were then reconstructed by implanting the tissue constructs.
Results: The intraoperative sites as well as the postoperative computerized tomographic scans demonstrated an accurate fit in the defect sites. The implanted scaffold constructs enriched with osteoblast-like cells were well tolerated and appeared to support bone formation, as revealed by histologic and immunohistochemical analyses.
Discussion: These results indicated that in vitro expanded osteoblast-like cells spread on a resorbable individualized scaffold can be capable of promoting the repair of bony defects in vivo.
Conclusion: These results warrant further attempts to combine computer modeling and tissue engineering for use in bone reconstructive surgery.

Keywords: animal model, bioengineering, bone, osteoblasts