Int J Oral Maxillofac Implants 19 (2004), No. 5 15. Sep. 2004
Int J Oral Maxillofac Implants 19 (2004), No. 5 (15.09.2004)
Histomorphometric Analysis of Homogenous Demineralized Dentin Matrix as Osteopromotive Material in Rabbit Mandibles
Pinheiro Carvalho, Valéria Abrantes / de Oliveira Tosello, Darcy / de Castillo Salgado, Miguel Angel / Gomes, Mônica Fernandes
Purpose: The aim of this work was to evaluate the effectiveness of homogenous demineralized dentin matrix (HDDM) slices in surgical bone defects created in the mandibles of rabbits and occluded with a polytetrafluoroethylene (PTFE) membrane in the promotion of bone growth.
Materials and Methods: Surgical bone defects were created in 36 adult rabbits and divided into 4 groups: bone defect (control), bone defect with PTFE membrane, bone defect with HDDM, and bone defect with both HDDM and a PTFE membrane (HDDM + PTFE). The rabbits were sacrificed after 30, 60, and 90 days, and the bone defects were examined histologically and by histomorphometric analysis (analysis of variance and the Tukey test).
Results: The volume of newly formed bone matrix was significantly greater in the HDDM and HDDM + PTFE groups than in the control and PTFE groups. The discrete inflammatory reaction found in the HDDM and HDDM + PTFE groups did not prevent the osteopromotive activity of the dentin matrix.
Discussion: HDDM slices were biocompatible and were resorbed during the bone remodeling process. They stimulated the newly formed bone until 30 days after implantation.
Conclusion: Bone repair was accelerated in the bone defects treated with HDDM in comparison to the control group.