We are using cookies to implement functions like login, shopping cart or language selection for this website. Furthermore we use Google Analytics to create anonymized statistical reports of the usage which creates Cookies too. You will find more information in our privacy policy.
OK, I agree I do not want Google Analytics-Cookies
The International Journal of Oral & Maxillofacial Implants
Login:
username:

password:

Plattform:

Forgotten password?

Registration

Int J Oral Maxillofac Implants 14 (1999), No. 2     15. Apr. 1999
Int J Oral Maxillofac Implants 14 (1999), No. 2  (15.04.1999)

Page 217-225


Effect of Transforming Growth Factor-ß on Osteoblast Cells Cultured on 3 Different Hydroxyapatite Surfaces
Ong, Joo L. / Carnes, David L.
In this study, the specific objective was to investigate the combined effect of different treatments of transforming growth factor-ß (TGF-ß) and hydroxyapatite (HA) on osteoblast response in vitro. Since the nature of bone cell responses in vitro is influenced by the properties of HA ceramics, this study was divided into 2 components: a chemical and crystallographic characterization of the HA ceramics, and an in vitro cell culture study. Sintered HA samples were observed to have the highest crystallite size, compared to as-received HA and calcined HA samples. No differences in surface roughness and chemical composition were observed between the sintered, calcined, and as-received HA surfaces. In concurrence with the x-ray diffraction, high-resolution x-ray photoelectron spectroscopy of Ca 2p also indicated a higher crystallinity on sintered HA samples compared to calcined and as-received HA samples. Protein production by osteoblast cells was not statistically different on the 3 HA surfaces in the absence of TGF-ß. However, there was a dose-dependent increase in TGF-ß-stimulated protein production on the 3 different HA surfaces. As indicated by increased alkaline phosphatase-specific activity, as well as 1,25 (OH2) vitamin D3-stimulated osteocalcin production, a more differentiated osteoblastlike phenotype was observed on the sintered HA surfaces compared to the as-received HA and calcined HA surfaces. An increased osteoblast-like cell activity on sintered HA surfaces in the presence of different TGF-ß dosage suggested that sintering of HA surfaces may play an important role in governing cellular response.

Keywords: alkaline phosphatase activity, heat treatments, hydroxyapatite, osteocalcin concentration, transforming growth factor-ß