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The International Journal of Oral & Maxillofacial Implants



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Int J Oral Maxillofac Implants 8 (1993), No. 3     1. May 1993
Int J Oral Maxillofac Implants 8 (1993), No. 3  (01.05.1993)

Page 264-272

Binding of Murine Osteoblastic Cells to Titanium Disks and Collagen I Gels: Implications for Alternative Interpretations of Osseointegration
Cooper, Lyndon F. / Handelman, Beverly / McCormack, SanM. / Guckes, Albert D.
Using 3H-thymidine-labeled MC3T3-E1 osteoblastic cells, the number of osteoblasts bound to titanium surfaces after various surface treatments and incubation periods was directly measured. MC3T3-E1 cell binding to titanium surfaces was saturable at a low level (approximately 10,000 cells/cm2). Although treatment of these surfaces with fibronectin, keratin sulfate, and the fibronectin-derived peptide GRGDS (glycine-arginine-glycine-glutamate-serine) increases cellular binding by 29% to 31%, the relative binding to titanium was 5 to 10 times lower than binding to collagen I gels. A collagen I matrix competed with the commercially pure titanium surfaces for cell binding from solution, suggesting that direct binding of osteoblasts to titanium surfaces present within an organic matrix may not be favored. The significance of immediate and direct bone cell attachment to titanium surfaces for osseointegration should be reevaluated.

Keywords: cell attachment, osseointegration, titanium