We are using cookies to implement functions like login, shopping cart or language selection for this website. Furthermore we use Google Analytics to create anonymized statistical reports of the usage which creates Cookies too. You will find more information in our privacy policy.
OK, I agree I do not want Google Analytics-Cookies
The International Journal of Oral & Maxillofacial Implants



Forgotten password?


Dear readers,

our online journals are moving. The new (and old) issues of all journals can be found at
In most cases you can log in there directly with your e-mail address and your current password. Otherwise we ask you to register again. Thank you very much.

Your Quintessence Publishing House
Int J Oral Maxillofac Implants 10 (1995), No. 1     1. Jan. 1995
Int J Oral Maxillofac Implants 10 (1995), No. 1  (01.01.1995)

Page 109-113

Stimulation of In Vivo Calcification Using Collagen Membranes Cultured with Osteoblastic Cells In Vitro: A Preliminary Report
Nagahara, Kuniteru / Mouri, Kenzou / Kanematsu, Nobutake / Shrestha, Prashanta / Meenaghan, Michael A.
Guided tissue regeneration (GTR) is a useful modality in the management of periodontal disease and for bone augmentation around osseointegrated implants. This study evaluated the in vivo use of atelocollagen membrane (AC) on which osteoblastic cells (OBCs) were cultured in vitro, for application as a GTR membranous material. Osteoblastic cells isolated in our laboratory from mouse calvaria formed a thin film on the AC in vitro which was easily manipulated after 21 days in culture. The AC and OBCs complex material (ACOB) was subjected to freezing and thawing and implanted in mouse subcutaneous tissue for the study of histologic events surrounding the implanted ACOB. Histologic findings in the subcutaneous tissue showed calcification on the ACOB at 28 days postimplantation, while no such finding was evident at the control site, where only AC without OBCs were grafted. The present study suggests the possibility of membrane calcification for GTR through ACOB produced by OBCs on an AC in vitro.

Keywords: atelocollagen, calcified material, culture, guided tissue regeneration, osteoblastic cell
fulltext (no access granted) Endnote-Export